Partially induced protection by a fraction of leishmania major promastigotes against murine leishmaniasis
IBJ-Iranian Biomedical Journal. 1998; 2 (1): 27-32
em Inglês
| IMEMR
| ID: emr-48109
ABSTRACT
Partially purified antigenic fractions of Leishmania major promastigotes were obtained by sodium dodecyl sulfate [SDS-PAGE] and electro-elution. Three isolated protein fractions designated as fractions [Fr.] 1, 2, and 3 correspond to 40-60, 60-80, 80-100 kilodalton [kDa] respectively. They were used for immunization of BALB/c mice against Leishmaniasis. The effects of these fractions on immune response of BALB/c mice against leishmanial infections was investigated by studying the infection course in infected mice, delayed type hypersensitivity [DTH] skin test, lymphocyte proliferation assay [LTT] in them. Subcutaneous immunization of mice with fraction 2 in conjugation with Complete Freund's Adjuvant [CFA] developed partial immunity against Leishmania major infection, and induced specific DTH response. Meanwhile this fraction exhibited no exacerbating effect on leishmanial infection course. Subeutaneous immunization with fraction 1 also induced partial protection in lesser extent than fraction 2 against leishmanial infection
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Leishmania major
/
Eletroforese em Gel de Poliacrilamida
/
Camundongos Endogâmicos BALB C
/
Antígenos de Protozoários
Limite:
Animais
Idioma:
Inglês
Revista:
Iran. Biomed. J.
Ano de publicação:
1998
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