Cloning and expression of thermus aquaticus DNA polymerase gene, using a thermo - inducible experession vector
IBJ-Iranian Biomedical Journal. 1998; 2 (2): 79-82
em Inglês
| IMEMR
| ID: emr-48116
ABSTRACT
DNA polymerase gene from Thermus aquaticus strain YT1 was amplified using VENTTH DNA polymerase and cloned under the control of lambda pr promoter and expression was induced by a shift in temperature. The culture was then sonicated, and after centrifugation the lysate was treated with polyethyleneimine followed by a salting-out step. Finally the protein was precipitated with ammonium sulfate and fractionated by gel filtration. The resulting enzyme preparation was stable and active
Buscar no Google
Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Temperatura
/
Thermus
/
Expressão Gênica
/
Reação em Cadeia da Polimerase
/
Vetores Genéticos
Idioma:
Inglês
Revista:
Iran. Biomed. J.
Ano de publicação:
1998
Similares
MEDLINE
...
LILACS
LIS