correlation between the presence of anti-HCV antibodies and HCV antigens in donors plasma, as determined by serological methods

ABSTRACT

Forty eight serum samples obtained from donor's and hepatitis patients, were included in this study to demonstrate the correlation between the antigens of the HCV and their antibodies in the plasma of the donors by using serological techniques. HCV-Ags were isolated and purified by using sucrose gradient technique, from serum samples positive for HCV-Ab. The HCV-Ags in serum samples and in fractions were detected by PHA assay, whereas, the antibodies Were detected by UBI.HCV EIA 4.0. The results indicates a 37.5% correlation beween the HCV-Ags and HCV-Ab is in the serum samples included in this study, also it indicate possibility of HCV transmission by routes other than that of blood transfusion and possiblity of finding a greater number of asymptomatic carriers. The results also demonstrates 70.8% of the samples were HCV positive whereas only 29.16% were HBV positive