Cellulolytic activity of thermomonospora fusca I. selection, characterization and determination of optimal growth conditions for maximal production of cellulase enzyme copmlex
Iraqi Journal of Microbiology. 1999; 11 (2): 71-90
em Inglês
| IMEMR
| ID: emr-50864
ABSTRACT
Thirty one thermophilic, celluloytic actinomycete isolates were selectively isolated from soil, compost, plant-waste biomass and ruminant manure samples, using Kosmachev liquid medium amended with 1% cellulose. Qulitative assessment of celluloytic acitivty was done measuring diameter of the clear zone by the growing bacterial colonies on soild medium containing cellulose. The relatively most active isolates were TA[10], TA[19] and TA[22]; subsequently were characterized and designated as Thermomonospora fusca Optimal growth conditions [time of incubation, temperature and pH] affecting cellulase complex production has been studied. Results showed that, optimal time for maxiarnl enzyme production was [192 216, 144]h. for isolates TA[10] and TA[22] respectively at an optimal temperature of [45°C], and pH values [8,9,9] respectively. Depletion of cellulose as the sole carbon and energy source, and the liberation of reducing sugars, were studied in relation with the cellular growth of the selected isolate TA[19], 810 mg/ml of reducing sugares were accumulated after 216h of incubation, with the concomitant consumption of 82% of cellulose after 240h. of growth
Buscar no Google
Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Actinobacteria
/
Celulases
Idioma:
Inglês
Revista:
Iraqi J. Microbiol.
Ano de publicação:
1999
Similares
MEDLINE
...
LILACS
LIS