Detection of hydatid antigen in human cyst fluid by reverse latex agglutination test

ABSTRACT

The present work studied the use of reverse latex agglutination [RLA] test in the diagnosis of CE as a test that could directly detect hydatid antigen in human cyst fluid samples. The results were reliable when compared with the direct microscopic examination of cyst fluid samples. Also, it was more reliable than indirect hemagglutination test as it showed 100% sensitivity. No false positive reaction was observed with samples from cystic tumors with a resultant specificity of 100%. Moreover, the test is easy to perform with a visually interpreted results within 2-3 minutes. The reagents are inexpensive, stable and easily available. These merits make the RLA test suitable for the diagnosis of CE, particularly in suspected cases with seronegative results or cases with sterile cysts