possible protective effect of some drugs on hepatic ischemia / reperfusion injury

ABSTRACT

This work aimed to clarify the possible protective effect of verapamil, allopurinol, dimethyl sulfoxide [DMSO], N-acetylcysteine [NAC], pentoxifylline [PTX] on hepatic injury induced by acute ischemia, followed by reperfusion in normal rats and rats sensitized to oxidative injury by depletion of endogenous glutathione. 84 male rats were randomized into seven groups. One served as a control group and the other groups were treated either with vehicle or with one of the previously mentioned drugs prior to induction of ischemia. The present study showed that ischemia/reperfusion [IR] significantly decreased hepatic glutathione [GSH] in both normal and GSH-depleted rat groups. All the tested drugs significantly prevented this reduction in both groups. Ischemia/reperfusion injury significantly increased hepatic malonyldialdehyde [MDA] level only in GSH-depleted rats. Hepatic myeloperoxidase [MPO] activity was significantly increased both in normal and GSH-depleted rats subjected to ischemia/reperfusion injury. Pretreatment with PTX significantly prevented this increase in hepatic MPO activity. Other tested drugs could not exert any protective effect