Histopathological examination and DNA ploidy in gastro-esophageal reflux disease

ABSTRACT

This study was carried out on 60 cases with typical symptoms of gastroesophageal reflux disease [GERD] with the aim of assessing the value of upper gastrointestinal endoscopy, histopathological examination [HPE] and DNA ploidy [probable number of chromosomes in the nucleus] in the diagnosis of gastroesophageal reflux disease [GERD] and its complications, especially the early detection of malignant transformation. Histopathological examination showed normal histological findings in eight patients [group I], chronic inflammation in forty patients [group II] and intestinal columnar metaplasia in twelve patients [group III]. DNA analysis revealed that all cases in group I had diploid DNA pattern. In group II, 26 patients had diploid DNA pattern and 14 patients had tetraploid pattern. In group III, six patients had diploid pattern, four patients had tetraploid pattern and two cases had aneuploid DNA pattern