Participation of angiotensin converting enzyme, prostaglandins and nitric oxide and possibility of their interaction in glycerol induced acute renal failure [A high microscopic, semi quantitative study]

ABSTRACT

This study was performed using adult male albino rats and acute tubular necrosis was induced by glycerol injection into the hind limbs. Inhibitors for angiotensin converting enzyme [ACE], prostaglandins [PG] and nitric oxide [NO] synthase were injected intraperitonealy before and after glycerol injection to study their roles in acute renal failure and the possibility of their interaction in the kidney in a similar pattern to that takes place in the heart. Tubular damage occurring was semi-quantified in kidneys of different groups. ACE inhibitor [perindopril] was found to reduce the glycerol-induced acute tubular necrosis establishing the injurious role of angiotensin converting enzyme in the pathogenesis of this condition. Injection of either PG synthesis inhibitor [voltaren] or NO synthase inhibitor [L-NAME] has caused increase of the glycerol-induced acute tubular necrosis indicating that both prostaglandins and NO has renoprotective effects. Injection of either PG synthesis inhibitor [voltaren] or the NO synthase inhibitor [L-N AME] together with the ACE inhibitor impairs its renoprotective effect denoting that prostaglandins and NO may mediate this renoprotection