Cloning and expression of acd genes of FMDV
Veterinary Medical Journal. 1999; 47 (3): 289-294
em Inglês
| IMEMR
| ID: emr-53052
ABSTRACT
RT-PCR amplification of VIA [3D] gene and protease [3C] using its primers. Legation of the PCR product with the cloning vector [using pET system]. Expression of the recombinant plasmid in E. coli expression host. Use of the expressed VIA protein in diagnosis of cattle infected with any of the 7 serotypes of FMD virus with the help of ELISA
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Bovinos
/
Ensaio de Imunoadsorção Enzimática
/
Aphthovirus
/
Clonagem de Organismos
Limite:
Animais
Idioma:
Inglês
Revista:
Vet. Med. J.
Ano de publicação:
1999
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