Influence of the state of dehydration and rehydration on the in-situ intestinal absorption of carotene in rats

ABSTRACT

This work aimed to evaluate the influence of an experimental model of diarrhea as well as the rehydration process on the in situ intestinal absorption of carotene in rats. Reproducible models of mild and severe infectious diarrhea were produced by peroral challenges with Salmonella typhimurium in adult male rats. The in situ intestinal absorption of carotene was evaluated along 90 minutes in cases of diarrhea in the presence and absence of oral rehydration solution as well as after rehydration. The utilized concentration of carotene was 0.83 mg%. Absorption was evaluated by determining the circulating fractions of carotene as well as the rate constants of absorption from the lumen to the intestinal membrane [K1] and from the intestinal membrane to the central compartment [K2]