Correlation between EMIT and GC/MS analysis of benzodiazepines in whole blood

ABSTRACT

The usefulness of using urine immunoassay kit for benzodiazepines screening in whole blood was evaluated by enzyme multiplied immunoassay technique [EMIT] Cobas Mira. Acetone extraction and rising of low cut-off calibrator to 70 ng/ml was done to avoid false positive results. Gas chromatography/mass spectrum analysis was performed for identification, quantification and confirmation of the results. Blood samples were collected from 48 benzodiazepines levels than GC/MS. Thirty samples of the estimated cases were positive, while 18 were negative. GC/MS analysis confirmed EMIT positive results and found that 12 cases of the negative samples were diazepam of low concentration and the remaining six negative cases were flunitrazepam i.e. GC/MS can identify the type of benzodiazepines