Study on the correlation between inoculum size, recovery of larvae using a new technique and some immunological parameters in experimental toxocariasis

ABSTRACT

In this study, 140 Swiss albino mice were inoculated with different infective doses [50, 200 and 1000] of T. canis eggs to study the effect of inoculum size on the distribution of recovered larvae from different organs [liver, lungs and brain] at two different times post infection [2 and 21 days pi] through two different techniques [the traditional acid digestion technique [ADT] and the new acid isolating technique [AIT]]. The results of the recovered larvae with AIT were comparable with those obtained with ADT to evaluate AIT versus ADT for recovering T. canis larvae. It was found that the mean number of the recovered larvae from the sampled organs from each of the three different inocula varied significantly indicating that inoculum size influenced the larval recovery. On day two pi using the different inoculum sizes, the recovery of larvae from liver and lungs has been occurred; while no larvae were detected in the brain. On day 21 pi using 50 eggs inoculum, no larvae were detected in the liver and lungs; while, on using the other two inocula, the recovery of larvae were obtained from all the sampled organs