Early diagnosis of mycosis fungoides by multiparametric image cytometry

ABSTRACT

In this study, image cytometry of DNA content, S phase and morphometric parameters were used as objective methods for the distinction between early mycosis fungoids [MF] and eczematous dermatitis. Ten cases of eczematous dermatitis and 20 cases of patch stage MF were studied. All the MF cases showed aneuploid histograms and all the eczematous dermatitis cases showed diploid histograms. A higher S phase fraction nuclear ploidy and DNA index and lower 2C fraction were detected in MF cases. All the 12 estimated morphometric parameters as well as the calculated nuclear contour index [NCI] were significantly higher in MF than eczematous dermatitis