Flavonoids and coumarins from hammada elegans [Bunge] Botsch and its biological activity

ABSTRACT

The phytochemical investigation of Hammada elegans [Bunge] Botsch family Chenopodiaceae, afforded six coumarins identified as scopoletin, scopolin, umbelliferone, xanthotoxol, isooxyimperatorin and esculetin, and two flavonoids [quercetin and quercetin 7-O- rhamnoside]. The identification of these compounds was achieved by m.p., UV, EI-MS, 1H-NMR, 13C-NMR and co-chromatography with authentic references. Quantitative estimation of total flavonoids using spectrophotometric method as well as mineral analysis were carried out. The anticoagulant activity of the ethanolic extract on rats showed a significant effect. The cytotoxic activity against human tumor cell lines U251 and MCF7 [brain tumor and breast carcinoma cell lines, respectively] proved no cytotoxic activity