Simultaneous high performance liquid chromatography determination of three sulfonylureas in plasma

ABSTRACT

A high-performance liquid chromatographic [HPLC] method has been developed for the simultaneous analysis of three frequently used sulfonylureas [chlorpropamide, glipizide and glibenclamide] by using a reversed phase C-8 column with a mobile phase consisting of 0.1% Ortho- phosphoric acid pH 2.7 isopropanol acetonitrile, [452530] operated at ambient temperature, with analysis time less than ten minutes. The eluted drugs were monitored by UV at 235nm. The extraction was performed by using dichloromethane after the plasma sample was mixed with the buffer [0.1% orthophosphoric acid].The overall recoveries and relative standard deviations were [93.7 +/- 5.3]% for chlorpropamide, [91.5 +/- 4.9]% for glipizide and [95 +/- 2.8]% for glibenclamide. The response was linear in the range [0.1 -100mg/ml] for chlorpropamide, glipizide, and glibenclamide, with r' > 0.999 for all drugs. Detection limits were 2ng/ml plasma for chlorpropamide, 15ng/ml plasma for glipizide and 7ng/ml plasma for glibenclamide, measured at a Signal/ Noise [S/N] of 3. No interference from administered drugs [barbiturates, b-blockers, Tranquillizer, Antihypertensive, Histamine antagonist, Antidepressant, anti emetic, and anticonvulsant] or endogenous constituents were observed