Indirect determination of lactose by atomic absorption spectrophotometry

ABSTRACT

An indirect atomic absorption method for the determination of lactose is developed. Heptavalent manganese is reduced in weakly acidic solutions by lactose to form insoluble MnO2. The concentration of lactose in the solution is determined from a calibration curve prepared from measurements made on known amounts of lactose. In the meantime the quantity of MnO2 formed is proportional to the concentration of lactose added and the formed Mn[2+] ions are determined by atomic absorption spectrophotometry