Comparison of urinary neopterin to creatinin ratio in multiple sclerosis patients and controlled group

ABSTRACT

Multiple Sclerosis as the most common cause of demyelinating disorders has a basis of autoimmunity. To describe a procedure to determine total and aromatic UNCR and their ratio as a marker of cell mediated immunity in MS patients and controlled groups. In this project UNCR was determined and compared in MS patients who reffered to the neurology department of Shariati's Hospital and normal subjects. Using HPLC and Novapak C18 [3.9x300mm] column at 25 degrees centigrade. Mean of aromatic UNCR were 337.6 +/- 47.2 and 1273.1 +/- 201.64 micromol/mol, mean of total UNCR were 484.64 +/- 60.2 and 1800.3 +/- 250.1 micromol/mol, and the ratio of aromatic/total UNCR were 0.664 and 0.73 in normal subjects and MS patients respectively. Prominent elevation of aromatic UNCR in MS patients in comparison with normal subjects is the sign of high activity of cell- mediated immunity in this group even though with treatment