Haemostatic and histopathological changes caused by alpha tocopherol and retinol palmitate

ABSTRACT

This work aimed to study the effect of alpha tocopherol [vitamin E] and retinol palmitate [vitamin A] on body weight, bleeding time, coagulation time, prothrombin time and kaolin partial thromboplastin of albino rat as well as to study the histopathological effect of these vitamins. A dose of 270 mg/kg/day of vitamin E and 500,000 IU/kg/day of vitamin A was given orally to albino rats for 21 days. The results showed that body weight was significantly decreased in both groups treated with vitamin E and vitamin A. It was found that vitamin E significantly increased the bleeding time and coagulation time, while vitamin A significantly increased the coagulation time only. However, the two groups treated with vitamin E and vitamin A showed a significant increase in prothrombin time, concentration and kaolin activated partial thromboplastin. The histopathological examination of liver, lung and kidney of autopsied animals revealed congestion, hemorrhage and mild inflammation, which were more severe in animals received vitamin E. In addition, the liver in both groups showed apoptosis and peripheral fatty changes