Studies on the induction and properties of 11-a-hydroxylasse from aspergillus nidulans
Egyptian Pharmaceutical Journal [National Research Center]. 2003; 1 (1): 25-33
em Inglês
| IMEMR
| ID: emr-61977
ABSTRACT
The induced cells of Aspergillus nidulans were homogenized with alumina and centrifuged for obtaining 11-alpha-hydroxylase. The results showed that the maximum 11-alpha-hydroxyprogetreone yield [98.5%] was obtained by using phosphate buffer at pH 7, transformation time 2 hr, at 35C, enzyme ratio [120] and Km value 6.15 mg/100 ml. The effect of some activators and inhibitors on 11-alpha-hydroxylase activity were also investigated. While, Mg+2, Co+2, Fe+2 and chloramphenicol appeared to be good inducers and Zn+2, Hg+2 and cyclohexamide showed different inhibitory effects. The 11-alpha- hydroxylase enzyme produced by A. nidulans was observed to be NADPH dependent
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Esteroides
/
Zinco
/
Biotransformação
/
Cobre
/
Ativação Enzimática
/
Inibidores Enzimáticos
/
Oxigenases de Função Mista
/
Hidroxiprogesteronas
/
Ferro
/
Magnésio
Idioma:
Inglês
Revista:
Egypt. Pharm. J. [NRC]
Ano de publicação:
2003
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