Up-regulation of the tumor suppressor gene P53 and WAF1 gene by IP6 in CP-3 human prostatic carcinoma cell line

ABSTRACT

This study investigated the effects of IP6 on the expression of p53 and WAF1/p21 in PC-3 human prostate carcinoma by immunocytochemistry. The immunocytochemical studies with anti p53 antibodies [wide type- PAb246 and Pab1620] and anti p21 WAF1 [EA10] antibodies demonstrated an increased level of p53 and p21 WAF1 after 3 and 6 days of treatment with 3.3 and 5 mM IP6. This increase was dose-dependent; however, a definite time-dependent increase was not observed. These data demonstrated that IP6 up-regulates the expression of the tumor suppressor gene p53 and p21 WAF1 gene and their modulation may be one of the mechanisms of the anti-neoplastic action of IP6 since loss of p53 function enhances cancer cells resistance to chemotherapeutic agents, the stimulating function of IP6 on p53 makes it an attractive adjuvant chemotherapeutic agent as well