Preparation, distribution, stability and tumor imaging properties of [62 Zn] bleomycin complex in normal and tumor - bearing mice

ABSTRACT

Bleomycin [BLM] has been labeled with radioisotopes and widely used in therapy and diagnosis. In this study BLM was labeled with [62Zn] zinc chloride for oncologic PET studies. The complex was obtained at the pH=2 in normal saline at 90°C in 60 min. Radio-TLC showed an overall radiochemical yield of 95-97% [radiochemical purity > 97%]. Stability of complex was checked in vitro in mice and human plasma/urine. Preliminary in vivo studies performed to determine complex stability and distribution of [62Zn] BLM in normal and fibrosarcoma-bearing mice. [62Zn] BLM accumulated significantly in induced fibrosarcoma tumors in mice according to bio-distribution/imaging studies. [62Zn] BLM can be used in PET oncology studies due to its suitable physico-chemical properties as a diagnostic complex in vitro and in vivo. Further studies should be performed for evaluation of the complex behavior in higher animals