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Cystatin capture-dot-enzyme-linked immunosorbent assay for immunodiagnosis and assessment of cure of experimental trichinellosis in mice
Journal of the Egyptian Society of Parasitology. 2003; 33 (1): 275-90
em Inglês | IMEMR | ID: emr-62841
ABSTRACT
Cystatin capture-dot-enzyme-linked immunosorbent assay [CC-dot ELISA] was evaluated as a new version of ELISA for the diagnosis of trichinellosis by the detection of anti-Trichinella spiralis cysteine proteinase [CyP] IgG using nitrocellulose membrane sensitized with cystatin as a capture reagent for CyP from T. Spiralis muscle larvae excretory secretary products [ESP] without purification compared with the detection of anti-T. spiralis IgG by conventional [conv.] ELISA using whole ESP. Experimentally infected mice with light [100 larvae/mouse] and heavy [300 larvae/mouse] infections by T. spirals larvae at 7, 14, 21 and 56 days post-infection and after flubendazole treatment were examined
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Índice: IMEMR (Mediterrâneo Oriental) Assunto principal: Triquinelose / Testes Imunológicos / Ensaio de Imunoadsorção Enzimática / Cisteína Endopeptidases / Sensibilidade e Especificidade / Larva / Camundongos / Anticorpos Limite: Animais Idioma: Inglês Revista: J. Egypt. Soc. Parasitol. Ano de publicação: 2003

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Índice: IMEMR (Mediterrâneo Oriental) Assunto principal: Triquinelose / Testes Imunológicos / Ensaio de Imunoadsorção Enzimática / Cisteína Endopeptidases / Sensibilidade e Especificidade / Larva / Camundongos / Anticorpos Limite: Animais Idioma: Inglês Revista: J. Egypt. Soc. Parasitol. Ano de publicação: 2003