Cystatin capture-dot-enzyme-linked immunosorbent assay for immunodiagnosis and assessment of cure of experimental trichinellosis in mice
Journal of the Egyptian Society of Parasitology. 2003; 33 (1): 275-90
em Inglês
| IMEMR
| ID: emr-62841
ABSTRACT
Cystatin capture-dot-enzyme-linked immunosorbent assay [CC-dot ELISA] was evaluated as a new version of ELISA for the diagnosis of trichinellosis by the detection of anti-Trichinella spiralis cysteine proteinase [CyP] IgG using nitrocellulose membrane sensitized with cystatin as a capture reagent for CyP from T. Spiralis muscle larvae excretory secretary products [ESP] without purification compared with the detection of anti-T. spiralis IgG by conventional [conv.] ELISA using whole ESP. Experimentally infected mice with light [100 larvae/mouse] and heavy [300 larvae/mouse] infections by T. spirals larvae at 7, 14, 21 and 56 days post-infection and after flubendazole treatment were examined
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Triquinelose
/
Testes Imunológicos
/
Ensaio de Imunoadsorção Enzimática
/
Cisteína Endopeptidases
/
Sensibilidade e Especificidade
/
Larva
/
Camundongos
/
Anticorpos
Limite:
Animais
Idioma:
Inglês
Revista:
J. Egypt. Soc. Parasitol.
Ano de publicação:
2003
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