Modified laser nephelometric technique to study RBC's aggregation stages

ABSTRACT

Laser nephelometric technique is developed to study RBC's aggregation. Using this aggregometer, blood aggregation curve is obtained from monitoring the scattering light signal from blood under various shear rates. From this curve, blood aggregation relaxation times [relaxation time of RBC's rouleaux formation T1 and time of network formation T2] and aggregation durability constant [beta] are determined. Results obtained indicate that as the shear rate increases the intensity of backscattered light increases due to increase in disaggregation. The relaxation time of formation of rouleaux [T1] was found to be correlated with HCT [hematocrit], RBC's count and HGB [hemoglobin]. Relaxation time of network formation [T2] and hydrodynamic durability constant [beta] have a weak correlation with hematological parameters