Immunophenotyping of childhood NHL in Iraq

ABSTRACT

Childhood NHL in Iraq was classified according to their cell of origin, using immunological markers available to be used in paraffin-embedded tissue sections. Thirty three children of both sexes from all parts of Iraq were included in this prospective study which was conducted for the period from December 1999- June 2001. Using streptavidin-biotin amplified immunoperoxidase immunostaining of paraffin-embedded tissue sections and different immunological markers specialized for T and B cells, CD30 and CD45. The results showed that all patient's biopsies were CD45 positive and most of the patients were of B-cells lymphoma, 9.1% of the patients were of T-cells lymphoma, and only 3.03% of the patients were of Ki-I positive large cell anaplastic lymphoma. We concluded that immunophenotyping of NHL are important factor for proper diagnosis and immunological characterization of childhood lymphomas, and also allows initiation of proper therapeutic approach as soon as possible