Ultrastructure immunohistopatholgical study on the anti-inflammatory response to dexamethasone under the influence of pyrethroids

ABSTRACT

The present study investigates the interaction of Dexamethasone with acute inflammation induced by Carrageenan [CGN] in the presence of the synthetic insecticide pyrethroid. Sixty adult male Sprague Dawly rats were utilized to study the immunological, histological and ultrastructure of the spleen, thymus gland and skeletal muscles. The animals were divided into six groups Control group, carrageenan-treated group, carrageenan-dexamathasone-treated group, and three insecticide exposed groups, one of them was exposed to insecticidal preparation only whereas the two other groups were treated with either CGN or CGN with dexamethasone. Microscopic examination of spleen and thymus gland parraffin sections of the CGN animals showed distortion of their normal architecture, degenerative changes and dilated and congested blood capillaries. Moreover, hyaline appearance, hemorrhage, cellular debris and necrosis could be observed. Extensive changes in the skeletal muscles were observed, while electron microscopic examination revealed irregular shaped nuclei and mitochondria. In addition insecticide-exposed CGN treated groups have shown microscopic degenerative changes both in spleen and thymus. These degenerative changes disappeared when treated with dexamethasone. Ultrastructure examination showed highly disintegrated myofilaments and completely degenerated mitochondria of the skeletal muscle. This study included also the determination of serum total protein, albumin, globulin and total immunoglobulin G, which recorded a disturbance in the immunoresponse, especially in the carrageenan and dexamethasone, pyrethroids-exposed groups