Evaluation of semi - quantitative PCR and IgG and IgM Elisa in Diagnosis of toxoplasmosis in females with miscarriage

ABSTRACT

This study was performed on 100 female [age 20-42 years] patients classified into group I, 40 patients presented with abortion in the first trimester; group II, 33 patients with abortion in the second trimester and group III, 27 patients with intrauterine fetal death [IUFD]. The positive percentages of semi-quantitative PCR and both IgG and IgM ELISA were 38% and 35%, respectively. Ten cases out of 38 were positive for toxoplasmosis by both PCR and ELISA-IgG, while 5 cases were positive by both PCR and ELISA-IgM. Sixteen cases out of 38 PCR positive cases were positive by both ELISA IgG and IgM. Sensitivity and specificity of both ELISA IgG and IgM were 81.57% and 93.54%, respectively. False negative results by ELISA were found in 7 out of 38 positive toxoplasmosis cases detected by semi-quantitative PCR. Three out of those seven cases with false negative by ELISA were detected with a trophozoite copy load of 101 trophozoite/mL in the blood sample by semi-quantitative PCR