Detection of genetic variabiltiy in non - human isolates of fasciola hepatica and Fasciola gigantica by the RAPD - PCR Technique

ABSTRACT

The present study showed the molecular characterization of Fasciola gigantica and F. hepatica isolates collected from cows and sheep, using the random amplified polymorphic DNA fragments-polymerase chain reaction [RAPDs-PCR] technique. Optimal standardization of amplification conditions and thermocyclation were made, using genetic markers. The methodology used compared the genetic patterns of the two species [interspecies] and inside each species [intra-species] between cow and sheep and the amplification fragments were between 135 and 741 base pairs of marker. The results showed genetic variations [polymorphisms] of Fasciola gigantica and F. hepatica with amplification fragment based on a 500-400 base pair [bp]. Inside each species, there were genetic variations in bovine and ovine and the amplification fragments were between 600 and 400 base pairs [bp]