Detection and cloning of desulfurization operon sox [dsz A and B] in rhodococcus FMF native bacterium

ABSTRACT

Native Rhodococcus FMF bacterium was selected among several isolated Iranian bacterium. Primary studies have shown that the bacterium use dibenzothiophene as a sulfur source. Since desulfurization is taken by a biochemical pathway, the present project was designed to detect, clone and sequence of desulfurization genes of the bacterium. Desulfurization operon was isolated from Rhodococcus erythropolis IGTS8 and was used as a probe for detection of desulfurization 4S pathway genes by southern blotting technique. Specific primers were designed and the dsz A and B genes were amplified by polymerase chain reaction [PCR]. PCR products were recovered from agarose gel and were cloned into the PTZ57R vector. In this study, recombinant PTZAB57R vector was constructed by the insertion of dsz A and B genes into the PTZ57R vector. The clones were confirmed by restriction digestion analysis. The PTZAB57R was extracted by large-scale method and was sequenced [MWG DNA Biotech]. Comparison of sequences of dsz A and B genes from native Rhodococcus FMF bacterium with Rhodococcus sp. IGTS8 bacterium were shown complete identity between them, which show that desulfurization pathway is conserved in this bacterium