Study of the exogenous effects of lysophosphatidyl choline on vascular and myocardial functions in isolated rat hearts and the influence of early ischemic preconditioning

ABSTRACT

The aim of this study was to examine whether ischemic preconditioning can prevent the vascular and cardiac dysfunction induced by exogenous lysophosphatidyl choline [LPC] perfusion in Langendorff perfused rat heart model. After equilibration, rats were randomly assigned into one of the following groups [n =8 per group] Group 1 [control] treated with vehicle for 15 minutes then hearts were perfused with normal krebs for 20 minutes; group 2 [LPC-treated], hearts of this group were perfused with LPC for 15 minutes then washed out for 20 minutes and group 3 [preconditioned group] same as in group 2, but hearts were preconditioned with one cycle of 10 minutes ischemic episode and 10 minutes reperfusion. The myocardial hemodynamic measurements [left ventricular developed pressure [LVDP], heart rate [HR], rate pressure product [RPP], end diastolic pressure [EDP] as well as GOT release activity] were measured after equilibration and at the end of each phase involved in this study. Coronary perfusion pressure [CPP] as a measure of coronary vasotone was also assessed. LPC is an important target when measures have to be taken to decrease the vulnerability to IR damage. It seems possible that ischemic preconditioning protect or improve IR damage on the basis of anti LPC action