Helicoacter pylori prevalence and resistance patterns in dyspeptic patients from Ismailia, Egypt

ABSTRACT

This study was aimed To determine the incidence of H. pylon with gastrointestinal disorders, to correlate its presence with endoscopic findings, and to determine the antibiotic resistance rates and patterns on isolates from dyspeptic patients in Ismailia, Egypt. Biopsy specimens were collected from 150 dyspeptic patients. One hundred and seven H. pylon isolates were isolated on selective Colombia blood agar and non-selective Brain heart infusion agar. Susceptibilities were determined for these isolates by disc diffusion methods. The prevalence of positive H. pylon isolates among the dyspeptic patients was 71.33%. A strong association was found between H. pylon and duodenal ulcer more than gastric ulcer and gastritis. The resistance rate to metronidazole, tetracycline, clarithromycin, and amoxicillin was 86%, 7.4%, 5.6%, and 0.9%, respectively. Dual resistance rate for metronidazole-tetracycline was 4.6% and for metronidazole-clarithromycin was 3.7%. The rate of triple resistance was 1.8% for metronidazoletetracycline-clarithromycin and 0.9% for metronidazole- tetracycline- amoxicillin It may concluded that Metronidazole resistance was a character of H. pylon isolates within most patients in Ismailia, while amoxicillin resistance is rare. Also combined resistance problem is growing due to the indiscriminate use of antibiotics. Antibiotics resistance monitoring is recommended continuously, since the successful treatment of H, pylon infection is becoming compromised by the emergence of resistance to antimicrobial. the disease