Effect of acrylamide on albino rat kidney histological, histochemical and electron microscopy studies

ABSTRACT

The effect of prolonged intake of acrylamide on the kidney of the albino rat was studied. Forty adult male albino rats were used in this study to evaluate the possible structural toxic effect of chronic intake of acrylamide. This substance was administered orally in three doses [0.01, 0.05 and 0.5 mg/kg b.w] three days weekly for six months. Examination of paraffin sections using the light microscope showed variable histological changes in the different study gps. The changes detected proceeded badly with increasing the dose. Some of the renal tubules lost their cytoplasmic brush borders as well as acidophilic granules. Widening of Bowman's space was noticed. Also, the interstitium and glomeruli showed lymphocytic infiltration and more congestions. Histochemical study revealed decreased glycogen and increased DNA contents. Electron microscopy studies showed aggressive changes in the nuclei, mitochondria and microvilli of kidney of rats [0.5 mg/kg]. These results indicated that chronic intake of acrylamide has a toxic effect on the kidney tissue cells when given with 0.5 mg/kg for six months as indicated by histological, histochemical and electron microscopic changes