Anti-hyperlipidemic effect and lipoidal constituents of grewia asiatica L. leaves

ABSTRACT

The aqueous ethanol extract, as well as, successive extracts of the dried powdered leaves of the plant were investigated for their anti-hyperlipidemic activity in induced-hyperlipidemic rats. The obtained data were statistically analyzed using Student's 't' test. Significant reduction in triglyceride and cholesterol levels were observed especially with the petroleum ether extract. Bioassay-guided fractionations have led to the fractionation of the petroleum ether extract by colunm chromatography. The hydrocarbon fraction was isolated and its components were identified by GC/MS analysis which revealed the identification of 50 compounds. Six triterpenes, two sterols, one diterpene and four fatty alcohol's were isolated and purified. They were identified by determination of their spectral data as [1] beta-amyrin [2] alpha-amyrin [3] alpha-amyrone [4] friedelin [5] glutin-5-en-3-ol [6] lanost-9 [11]-en-12-one [7] stigmast-7-en-3-ol [8] beta- sitosterol [9] phytol [10] docosanol [11] hexacosanol [12] heptacosanol and [13] nonacosanol. The fatty acids fraction was prepared, methylated and its components were identified by GC/MS analysis which revealed the identification of 16 compounds. It is worth to mention that the antihyperlipidemic activity test, as well as, the isolation of the identified compounds from G. asiatica leaves are carried out for the first time in the present study