Iranian Journal of Public Health. 2005; 34 (4): 58-66
em Inglês
| IMEMR
| ID: emr-71135
ABSTRACT
The human erythrocyte is a rich raw material for the purification of Cu-Zn superoxide dismutase [SOD]. We applied a simple and rapid procedure for the purification of SOD from human erythrocytes by ion exchange chromatography. The purified SOD had a specific activity of 2285.6 u/mg protein and gave a single band on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate [SDS] and each of its to subunit has a molecular weight about 18600 daltons [SOD molecular weight is 37200 daltons].The physicochemical properties of the enzyme obtained by this method are identical to those of the native protein.This procedure appears, therefore, to be a convenient and easily method for isolating this enzyme
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IMEMR (Mediterrâneo Oriental)
Assunto principal:
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Idioma:
Inglês
Revista:
Iran. J. Public Health
Ano de publicação:
2005
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