[Anti-tumor evaluation of adenosine and cyclohexyladenosine [A1 receptor selective agonist] using potato disc assays]

ABSTRACT

In this study, adenosine and cyclohexyladenosine [CHA] were tested for possible anti-tumor activity using potato disc assays. MIC of adenosine and CHA were determined using microplate method. In potato disc assay, discs were cut of potato with specific diameter and transferred on 1.5% agar under a laminar air cabinet. 50 ?l of a mixture containing suspension of Agrobacterium tumefaciens and the solution of agents were inoculated on potato discs in plates. The plates were incubated in 25 °C for 21 days until the tumors were counted. Adenosine and CHA did not show any anti-bacterial activity. The IC50 values of adenosine and CHA against tumor were 93.78 and 19.56 microM, respectively. DPCPX [A1 selective adenosine receptor antagonist] inhibited the anti-tumor activity of adenosine and CHA, while itself did not show any effect against potato disc tumors. This study indicates that adenosine and CHA have anti-tumor activity, and this effect is mediated via A1 receptor exists in potato cell or A. tumefaciens