Determination of flavonalignans of dried fruits of silybum marianum [L.] gaertn collected from different areas of Iran by spectrophotometry, TLC and HPLC

ABSTRACT

Silymarin complex consisted of five flavonolignans [silybum A and B Isosylibin A and B, silychristin, silydianin and taxifolin] isolated from dried fruits of Silybum marianum [L]Gaertn silybin is the main component of silymarin complex. Hepatoprotective activity of silymarin may be related to its antioxidant property. This study was conducted to understand the environmental condition on silymarin methabolism. Silymarin extraction and flavolignans assay by spectrophotometery, TLC and HPLC was performed on milk thistle seeds collected from different areas of Iran, Hungarian seeds cultivated in greenhouse and field in Karaj. Valasht and Borazjan had the highest levels of silymain content by spectrophotometery method. In TLC method all 5 component were identified. We analyzed the flavolignans contents by HPLC and results showed that borazjan had the highest level of silymarin. To produce commercially silymarin we need to search for the best genotype