Characterization of specific trichomonas vaginalis target antigens from different isolates by immuno-blotting against hyperimmune rabbit serum
Journal of the Egyptian Society of Parasitology. 2005; 35 (3): 891-898
em Inglês
| IMEMR
| ID: emr-72378
ABSTRACT
The SDS-PAGE and immunoblot methods were used to identify Trichomonas vaginalis specific target antigen[s]. Ten T. vaginalis isolates, cultured on TYM media, were analyzed by Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis [SDS-PAGE], revealed 34 distinct bands. Immunoblotting against a hyperimmune rabbit serum, showed 20 reactive bands ranging from 14-205 kDa. There was isolate to isolate variability among 8 isolates, while 2 isolates [6, 7] showed a similar antigenic patterns. Imunoblotting of the isolates showed a total of 20 molecular weight bands, 80% of isolates gave positive immunologic reactions above 100 kDa, while below 100 Kda all the isolates recognized the different molecular weight bands. Out of 20 reacting bands, 5 main bands were detected, 29, 66, 84, 95 and 115 kDa gave positive percent of 60, 60, 60, 90 and 80% among the 10 isolates respectively
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Coelhos
/
Immunoblotting
/
Eletroforese em Gel de Poliacrilamida
/
Animais de Laboratório
/
Antígenos
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Inglês
Revista:
J. Egypt. Soc. Parasitol.
Ano de publicação:
2005
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