Improvement in sperm motility viability and normality after treatment with prednisolone, antisperm antibody separation and in vitro sperm activation in immunologically infertile men

ABSTRACT

Antisperm antibody [ASA] as a cause of men infertility was fist reported in 1954. Different assays were developed for the detection of scrum-bound antibodies. The objective of this study is to study the effect of prednisolone, antisperm antibody separation [ASAS] and in vitro sperm activation on sperm motility, viability and morphology in immunologically infertile patients. Semen samples of 250 immunologically infertile patients were examined by seminal fluid analysis and microagglutination test to check the presence of sperm agglutination and antisperm antibodies [ASA]. The patients received 5mg prednisolone three times per day for two weeks then the dose was reduced to two tablets per day for four days and further reduced to one tablet for three days followed by one week of rest period. The treatment regimen was continued for three months and seminal fluid analysis was performed before and after the treatment. Sperm motility percent, sperm grade activity, sperm motility index, normal sperm morphology and sperm viability after the treatment were significantly improved [P< 0.001] while the percent of sperm agglutination, shaky head sperm movement percent of abnormal sperm morphology were significantly decreased [P < 0.0001]. the progress in the improvement of sperm quality was started at the end of 4[th] week after treatment and increased gradually up to the end of the 12[th] week. Application of prednisolone therapy, ASAS technique and in vitro sperm activation found to be effective and resulted in significant improvement sperm quality. These active and viable sperm following treatment may be used for intrauterine insemination or in vitro fertilization [IVF] and embryo transfer [ET] for the treatment of immunological infertile men