Coxsackie B Viruses as the main etiologic agent of myocarditis: Characterization of capsid proteins
New Egyptian Journal of Medicine [The]. 2005; 32 (Supp. 3): 7-15
em Inglês
| IMEMR
| ID: emr-73848
ABSTRACT
Enteroviruses especially Coxsackie B viruses [CBVs] are responsible for approximately 50% of viral myocarditis cases. Coxsackie B viruses were inoculated in BGM cells for three successive passages. Viral capsid proteins were screened by SDS-PAGE, followed by Western blot. Electrophoretically separated proteins of CB2, CB4 in comparison to CA9, Echo9, and PV1 Sabin, showed common peptides particularly at the low molecular weight [ranging between 40 to 10 kDa]. The results of immunoblotting analysis using polyclonal antibodies raised against the whole Coxsackieviruses B viral particles did not reflect that all those bands are immunogenic, nevertheless those peptides could be used to raise specific antibodies which in turn could be useful in detecting viral circulating antigens
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Western Blotting
/
Enterovirus Humano B
/
Infecções por Coxsackievirus
/
Proteínas do Capsídeo
/
Eletroforese em Gel de Poliacrilamida
/
Anticorpos Monoclonais
Idioma:
Inglês
Revista:
New Egypt. J. Med.
Ano de publicação:
2005
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