Improved high performance liquid chromatographic analysis of diltiazem in human plasma
PAFMJ-Pakistan Armed Forces Medical Journal. 2005; 55 (3): 214-218
em Inglês
| IMEMR
| ID: emr-74045
ABSTRACT
A simple high-performance liquid chromatographic method was developed for determination of diltiazem in human plasma. Diltiazem and the internal standard, verapamil, were extracted from plasma samples using mixture of n-hexane and diethyl ether. The mobile phase was 0.1M ammonium dihydrogen phosphate - acetonitrile [6238 v/v]. Triethylamine [0.08% v/v in the mobile phase] was added before the pH was adjusted to 5.9 with 85% phosphoric acid. Analysis was run at a flow rate of 1.0 ml/min at a detection wavelength of 238 nm. The method was specific and sensitive with a detection limit of 2.5 ng/ml at a signal-to-noise ratio of 31. The limit of quantification was set at 5 ng/ml. The calibration curve was linear over a concentration range of 5-160 ng/ml. Mean recovery value of the extraction procedure was about 90%, while the within and between day coefficient of variation and percent error values of the assay method were less than 10%
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Plasma
/
Diltiazem
/
Verapamil
/
Sensibilidade e Especificidade
/
Angina Pectoris
Limite:
Humanos
Idioma:
Inglês
Revista:
Pak. Armed Forces Med. J.
Ano de publicação:
2005
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