Prevalence and sensitivity of different strains of bacteria; Special reference to MRSA in a surgical unit of MAYO Hospital Lahore

ABSTRACT

The correct registration, reporting and analysis of the resistance situation within a hospital is the first step in halting the emergence of antibiotic resistance, we therefore decided o analyze prevalence of bacteria and current trends of antibiotic resistance within our hospital. Orthopaedic ward of Mayo Hospital Lahore. Nov 2003 to Apr 2004. Isolates were taken from 157 patients admitted in Orthopaedic ward of Mayo Hospital Lahore during 6 months period randomly. The samples were collected from patient's wounds, the patients were not given any antibiotics 48 hours prior to collection of the specimen. Isolates were also taken from the environment [Rooms, AC ducts, corridors of the wards, operation theatre] and OT instruments. Isolates were inoculated on standard media in optimum environment and standard internationally accepted methods were applied for identification of bacteria. We found out that the most prevalent bacterium to be staphylococcus aureus 33 [1 8.75%] and seven were MRSA susceptible to Amikacin only. Where as the other bacteria pseudomonas auregenosa 38 [21.59%], E. Coli 19 [10.79%]. Proteus 14 [7.95%]. Streptococcus pyogenes 10 [5.68%], enterobacter 8 [4.55%], Klebsiella 5 [2.84%] more or less showed sensitivity to Amikacin. No growth was seen in 36 cases From among the 50 evironmental isolates, Bacillus 18 [36%] Staphylococcus in combination with bacillus 23 [46%] and No growth in 9 [18%]