Detection of schistosoma haematobium egg antigen by western blot

ABSTRACT

Eggs of Schistosoma release a potent soluble egg antigen SEA" which is used as an important marker in immunodiagnosis of schistosomiasis. Electrophoresis and identification of different protein fractions of the antigen are used in accurate serological diagnosis. This work aimed to detect Schistosoma haematobium egg antigens in human serum and hyperimmune sera of rabbits by Western blot [WB]. The results revealed important bands detected with rabbit anti-sera against [SEA] at 32-34, 40, 63 and 100 KD. In case of human anti-sera, the most important bands were at 24-26, 32-34, 40, 63 110 and 150 KD. This work was done for the first time in Egypt and its results agreed with what was done before by other methods: