role of L-type calcium channels in the vascular effect of trigonella foenum-graecum L. in diabetic rats

ABSTRACT

Some ion channels like voltage-operated calcium channels [VOCC] within the plasma membrane of vascular muscle cells from the walls of resistance arteries and arterioles play a central role in the regulation of vascular tone. On the basis of reports about the beneficial attenuating effect of fenugreek [Trigonella foenum-graecum L.; TFG] on the contractile reactivity of aortic rings of diabetic rats, this study was carried out to evaluate the possible involvement of L-type voltage-operated calcium channels in the vascular effect of this medicinal plant. For this purpose, male Wistar rats were made diabetic using streptozotocin [STZ, 60 mg/Kg, i.p]. The extract-treated control and diabetic rats received aqueous leaf extract of TFG [200 mg/Kg, i.p.] every other day for two months. At the end of the study, contractile response of isolated aortic rings to KC1 and noreadrenaline [NA] was determined in the absence and presence of the calcium channel blocker nifedipine. The results showed that aortic rings from diabetic rats are more responsive to the effect of KC1 and NA than those of controls, TFG extract treatment could attenuate the enhanced contractile response of aortic rings of diabetic rats, and nifedipine pretreatment could partially neutralize the beneficial effect of this extract. It is concluded that TFG extract attenuates the enhanced vascular reactivity in chronic diabetic rats and voltage-operated calcium channels are in part responsible for this effect of TFG extract