Relationship between Gleason's score and immunohistochemical staining intensity of tissue PSA in prostatic carcinoma

ABSTRACT

To evaluate the usefulness of Immunohistochemical Staining of Tissue PSA in determining the severity of Prostatic Carcinoma. Material and An explanatory study carried out at the Department of Histopathology of a Tertiary Care Hospital Lahore, from January 1999 to July 2002. Sixty-two [62]- cases of prostatic adenocarcinoma were graded with Gleason's score and stained with immunohistochemical stain for tissue PSA. Intensity of staining was correlated with Gleason's score and relationship was evaluated by ANOVA and simple regression. Specificity and sensitivity of staining intensity was estimated for specific Gleason's scores. Age of the 62 cases ranged between 50 ' 90 years and no significant difference was found between age groups regarding tumor cell differentiation. Staining intensity was found to be positively related to cellular differentiation [->2 51]. 'Relationship between staining intensity and Gleason's score showed an inverse relationship with ANOVA / linear regression; F statistic of 209.72 [p.0001] and R 0.882. Our study confirmed the findings of other international studies exploring relationships between Gleason's score and staining intensity of tissue PSA. Staining of tissue PSA with immunohistochemical stains is helpful in diagnosing the severity of the tumor grade as a complimentary tool along with Gleason's scoring