Serological tests and biochemical profiles in camels infected with brucellosis

ABSTRACT

Blood samples were collected from 80 camels kept in closed farm [Camel production unit Animal production Institute and others], another 72 blood samples were collected from camels kept in close contact with cattle and other small ruminant from different areas in Gize governorate. As well as 94 blood samples were collected from imported camel from Sudan in camel market. Our data showed that the highest percent of positive reactors was observed in the imported camels [Sudanese camel] in large herd [8.50 to 11.70% [9.50%, 10.60%. 9.50%, 8.51%. 9.57% and 11.70 to 8.50% for RBPT, BAPA, Riv, SAT, MET and DI/ respectively], Camels in contact with other animals [6.94 to 11.10% [8.30%, 9.40%, 8.30%, 6.94%, 8.33% and 8.30 to 11.10% for RBPT, BAPA, Riv, SAT, MET and DIA, respectively] and Camels in closed farms [0.00 to 2.50% [1.25%, 2.50%, 0.00%, 1.25%, 0.00% and 1.25 to 5.00% for RBPT. BAPA, Riv, SAT, MET and DIA, respectively]. The results of sensitivity and specificity of DIA revealed that DIA using n-lauroylsarcosin extract is more specific than DIA whole bacterial antigen. The sera of infected camels with brucclla [either camels contact with animals or imported camels] showed elevated levels in each of the GGT, LDH, ALP, AST, ALT, total protein, albumin, glucose, urea, uric acid and creatinine. The sera of imported camels infected with brucellosis were characterized by increased levels of protein bands with molecular weights 29.83 -30.11, 45.95-46.27 kDa, with increase of 34.64, 35.29, 74.67, 87.74, 98.96, 99.75, 104.62, 110.57, 115.54, 132.63, 134.12, 138.69, 140.25 kDa protein bands in both camels contact with animals and imported camels infected with brucellosis. Protein bands 181.3,1-183.34 and 214.36 KDa were apparent in camels contact with animal's sera infected with brucellosis and protein bands 189.59 and 231.79 were present in imported camel's sera infected with brucellosis especially in 1/320 antibody sera. The LDH and ALP iso-enzymes had a characteristic profile in brucellosis. Our conclusions that imported camels infected brucellosis followed by camels contact with animals infected with brucellosis had more serious biochemical discordance. The results give us an index to diagnosis of brucellosis in the imported camel. The incidence percent of brucellosis in camels in closed farm in dedicate importance, good prognosis mean and diagnosis tools to detect and eliminate the infected camels, and aid in epidemiological controls of the disease