Characterization of a chitinase gene encoded by virus-sensitive chlorella strains and expressed during virus infection
Arab Journal of Biotechnology. 2007; 10 (1): 81-96
em Inglês
| IMEMR
| ID: emr-81810
ABSTRACT
A chitinase gene [hChti-1] was found and characterized in a few strains of Chlorella species that are sensitive to Chlorella viruses. A cDNA clone of hChti-1 obtained from Chlorella strain NC64A contained a 401 aa [1206 bp] open reading frame, whose amino acid sequence was most similar to that of family 18 catalytic domain I of dual-structured chitinase vChti-1 encoded by Chlorella virus CVK2. The expression of hChti-1 was induced by virus infection and functional chitinase protein was produced. Since the hChti-1 gene was strictly limited to virus-sensitive strains, its occurrence seems to be involved in the virus infection mechanism. This is the first report of chitinase encoded and produced by unicellular algae
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Sequência de Bases
/
Chlorella
/
Northern Blotting
/
Reação em Cadeia da Polimerase
Idioma:
Inglês
Revista:
Arab J. Biotechnol.
Ano de publicação:
2007
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