simple and reliable PCR-based method for detection and screening of transgenic plants transformed with the same endogenous gene

ABSTRACT

To detect integration of a transgene in genetically modified plants that have a native copy of the gene, a simple and reliable PCR-based method has been established. This method is then used to screen for transgenic wheat plants that have been transformed with the high molecular weight glutenin subunit Dy10 gene. Two PCR primers were designed, the forward one matched upstream flanking nucleotide sequences in the pBlueScript KS vector and the reverse primer hybridizes with a Dy10 specific. Use of these primers produced a distinguishable marker only from the transgene with no appreciable signal detected from the endogenous gene