Detection of chlamydia trachomatis in endocervical specimens by an enzyme- linked polymerase chain reaction assay
DARU-Journal of Faculty of Pharmacy Tehran University of Medical Sciences. 2007; 15 (2): 100-104
em Inglês
| IMEMR
| ID: emr-82122
ABSTRACT
Chlamydia trachomatis [CT] is the most common cause of sexually transmitted infections [STI] worldwide and its early detection and treatment can reduces the high morbidity associated with this infection. In this study a sensitive diagnostic polymerase chain reaction [PCR]-based enzyme immunoassay [PCR-EIA] method was developed which detects CT in women with cervicitis. Endocervical swabs collected from 123 women [20-55 years] with cervicitis were tested by both conventional PCR, and PCR-EIA assays, using identical sets of primers to amplify a CT-specific plasmid. For the conventional PCR, amplicons were detected by agarose gel electrophoretic analysis and the PCR-EIA assay used biotin-labeled primers, strepavidin-coated plates, a digoxigenin-labeled probe, and a final enzyme-linked colorometric analysis [405 nm] was used to measure the CT amplicon. The frequency of positive CT infection by conventional PCR and PCR-EIA assay was 7% and 17%, respectively. The highest frequencies of CT infection were among women of 31-40 years old group [25%]. The PCR-EIA limit of detection, calculated by linear regression analysis, was10 pg of CT DNA [r[2]=0.9642]. The degree of agreement [Kappa] between the conventional PCR and PCR-EIA method was 0.556 [p<0.0001]
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Infecções Sexualmente Transmissíveis
/
Cervicite Uterina
/
Colo do Útero
/
Técnicas Imunoenzimáticas
Tipo de estudo:
Estudo de rastreamento
Limite:
Feminino
/
Humanos
Idioma:
Inglês
Revista:
J. Fac. Pharm. Tehran Univ. Med. Sci.
Ano de publicação:
2007
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