Post treatment effect of Grewia asiatica against radiation induced biochemical changes in brain of Swiss albino mice

ABSTRACT

The aim of the present study was to evaluate the radioprotective effect of Grewia asiatica fruit pulp extract [GAE] on Swiss albino mice exposed to gamma radiation. In the present study radioprotective efficacy of Grewia asiatica [rich in anthocyanin, carotenes, vitamin C, etc.] was studied against radiation induced biochemical alterations in mice cerebrum. For experimental study, healthy Swiss Albino mice were selected from an inbred colony and divided into four groups. Group I [normal] did not receive any treatment. Group II was orally supplemented [GAE] once daily at the dose of 700 mg/kg.b.wt/day for fifteen consecutive days. Group III [control] received distilled water orally equivalent to GAE for fifteen days than exposed to 5 Gy of gamma radiation. Group IV [IR+Drug] was administered orally [GAE] for 15 consecutive days once daily after exposed to single dose of 5Gy of gamma radiation respectively. Mice were sacrificed at different autopsy intervals viz. 1, 3, 7, 15 and 30 days and brain were removed for various biochemical estimations viz. glutathione [GSH], lipid peroxidation [LPO] and protein. GAE post treatment renders protection against various biochemical changes in mice brain. Radiation induced augmentation in the levels of LPO was significantly ameliorated by GAE post-treatment. Radiation-induced depletion in the level of GSH, protein was checked significantly by GAE administration. These results indicate that Grewia asiatica fruit extract [GAE] is able to protect the brain of Swiss albino mice against radiation induced biochemical alterations