Association of the expression of IL-4 and IL-13 genes, IL-4 and IgE serum levels with allergic asthma
Iranian Journal of Allergy, Asthma and Immunology. 2007; 6 (2): 67-72
em Inglês
| IMEMR
| ID: emr-83119
ABSTRACT
Immune and inflammatory responses mediated by cytokines, play important roles in the pathophysiology of asthma. These responses are associated with overexpression of Th2 cytokines such as IL-4 and IL-13. These two cytokines use common receptors for signaling that lead to identical immunological effects and regulation of the Th1/Th2 balance. The aim of this study was to determine whether patients with allergic asthma display overexpression of IL-4 and IL-13 genes. Using RT-PCR, we examined the expression of IL-4 and IL-13 genes in twenty asthmatic cases and twenty normal individuals. Total levels of serum IgE and IL-4 were also determined by ELISA method. Expression of IL-13 gene in 70% of patients with allergic asthma was higher than controls [P=0.01]. There was no correlation between the expression of IL-13 gene and total level of serum IgE [P=0.07]. Expression of IL-4 gene was detected in 30% of the patients and none of the normal individuals as determined by RT-PCR [P=0.01]. Mean of serum IgE levels in patients and controls were 84.9 IU/ml and 62.2 IU/ml, respectively. Level of serum IgE was more than 100 IU/ml in 30% of patients [P=0.03]. Mean of serum IL-4 levels in patients and controls were 15.73 pg/ml and 13.07 pg/ml, respectively. There was a relation between levels of serum IgE and IL-4 in 73% of cases. The results showed that there was a correlation between the expression of IL-4 gene and the level of serum IL-4. Levels of serum IgE and IL-4 were considerably higher in asthmatics than nonasthmatic controls
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Asma
/
Reação em Cadeia da Polimerase
/
Interleucina-4
/
Interleucina-13
/
Eletroforese
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Iran. J. Allergy Asthma Immunol.
Ano de publicação:
2007
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