[Effect of antioxidants supplementation on human sperm parameters after freezing]

ABSTRACT

Antioxidant reduces oxidative stress during cryo-preservation. The aim of this study was to find out the effects of vitamin E and C on sperm parameters after cryo-preservation. Human semen samples were obtained from Vali-e-asr Hospital. The samples were divided in two groups [normal and oligospermia groups]. Semen was pooled in liquid nitrogen after thawing, samples were centrifuged, then vitamin E and C were added to medium and the aliquots were incubated for 45 minutes in incubator Co2. In control group, no antioxidant was added to medium. Sperm parameters were analyzed according to WHO criteria. Data was analyzed by ANNOVA test. There was a significant increase in the progressive motility and viability of sperm which was supplemented by vitamin E, with 1, 2 Mm [p<0.05] in the normal groups [the increase in the oligospermia group, after addition of vitamin E with 1, 2, Mm was not significant]. Vitamin C did not have a significant effect on sperm parameters with 1, 2 Mm concentration. Supplementation of media with alpha-tocopherol is beneficial for sperm motility and viability rates after cryopreservation and it may be of clinical value in assisted conception procedures